A filtration-based protocol to isolate human plasma membrane-derived vesicles and exosomes from blood plasma
The methods of Plasma Membrane-derived Vesicle (PMV) isolation and quantification vary
considerably in the literature and a new standard needs to be defined. This study describes
a novel filtration method to isolate PMVs in plasma, which avoids high speed centrifugation,
and to quantify them using a Becton Dickinson (BD) FACS Calibur™ flow cytometer, as
annexin V-positive vesicles, larger than 0.2 μm in diameter. Essentially microvesicles (which
comprise a mixture of PMVs and exosomes) from citrate plasma were sonicated to break up …
considerably in the literature and a new standard needs to be defined. This study describes
a novel filtration method to isolate PMVs in plasma, which avoids high speed centrifugation,
and to quantify them using a Becton Dickinson (BD) FACS Calibur™ flow cytometer, as
annexin V-positive vesicles, larger than 0.2 μm in diameter. Essentially microvesicles (which
comprise a mixture of PMVs and exosomes) from citrate plasma were sonicated to break up …