Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

Z Wang, H Wu, D Fine, J Schmulen, Y Hu, B Godin… - Lab on a Chip, 2013 - pubs.rsc.org
Z Wang, H Wu, D Fine, J Schmulen, Y Hu, B Godin, JXJ Zhang, X Liu
Lab on a Chip, 2013pubs.rsc.org
We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous
silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can
preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and
cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous
nanowires in PBS buffer.
We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.
The Royal Society of Chemistry