Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: selective externalization of the receptor

BT Pan, RM Johnstone - Cell, 1983 - cell.com
BT Pan, RM Johnstone
Cell, 1983cell.com
The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been
followed using FITC-and '251-labeled anti-transferrin-receptor antibodies. Vesicles
containing peptides that comigrate with the transferrin receptor on polyacrylamide gels are
released during incubation of sheep reticulocytes, tagged with anti-transferrin-receptor
antibodies. Vesicle formation does not require the presence of the anti-transferrin-receptor
antibodies. Using 1251-surface-labeled reticulocytes, it can be shown that the '251-labeled …
Summary
The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC-and ‘251-labeled anti-transferrin-receptor antibodies. Vesicles containing peptides that comigrate with the transferrin receptor on polyacrylamide gels are released during incubation of sheep reticulocytes, tagged with anti-transferrin-receptor antibodies. Vesicle formation does not require the presence of the anti-transferrin-receptor antibodies. Using 1251-surface-labeled reticulocytes, it can be shown that the ‘251-labeled material which is released is retained by an immunoaffinity column of the anti-transferrin-receptor antibody. Using reticulocytes tagged with 1251-labeled anti-transferrin-receptor antibodies to follow the formation of vesicles, it can be shown that at 0% or in phosphate-buffered saline the rate of vesicle release is less than that at 37% in culture medium. There is selective externalization of the antibody-receptor complex since few other membrane proteins are found in the extemalized vesicles. The anti-transferrin-receptor antibodies cause redistribution of the receptor into patches that do not appear to be required for vesicle formation.
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