[HTML][HTML] The effect of long term calorie restriction on in vivo hepatic proteostatis: a novel combination of dynamic and quantitative proteomics
Calorie restriction (CR) promotes longevity. A prevalent mechanistic hypothesis explaining
this effect suggests that protein degradation, including mitochondrial autophagy, is
increased with CR, removing damaged proteins and improving cellular fitness. At steady
state, increased catabolism must be balanced by increasing mitochondrial biogenesis and
protein synthesis, resulting in faster protein replacement rates. To test this hypothesis, we
measured replacement kinetics and relative concentrations of hundreds of proteins in vivo in …
this effect suggests that protein degradation, including mitochondrial autophagy, is
increased with CR, removing damaged proteins and improving cellular fitness. At steady
state, increased catabolism must be balanced by increasing mitochondrial biogenesis and
protein synthesis, resulting in faster protein replacement rates. To test this hypothesis, we
measured replacement kinetics and relative concentrations of hundreds of proteins in vivo in …