[HTML][HTML] Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest
KB Sylvestersen, H Horn, S Jungmichel… - Molecular & Cellular …, 2014 - ASBMB
The covalent attachment of methyl groups to the side-chain of arginine residues is known to
play essential roles in regulation of transcription, protein function, and RNA metabolism. The
specific N-methylation of arginine residues is catalyzed by a small family of gene products
known as protein arginine methyltransferases; however, very little is known about which
arginine residues become methylated on target substrates. Here we describe a proteomics
methodology that combines single-step immunoenrichment of methylated peptides with high …
play essential roles in regulation of transcription, protein function, and RNA metabolism. The
specific N-methylation of arginine residues is catalyzed by a small family of gene products
known as protein arginine methyltransferases; however, very little is known about which
arginine residues become methylated on target substrates. Here we describe a proteomics
methodology that combines single-step immunoenrichment of methylated peptides with high …