A guide to choosing fluorescent proteins

NC Shaner, PA Steinbach, RY Tsien - Nature methods, 2005 - nature.com
NC Shaner, PA Steinbach, RY Tsien
Nature methods, 2005nature.com
The recent explosion in the diversity of available fluorescent proteins (FPs),,,,,,,,,,,,,,,
promises a wide variety of new tools for biological imaging. With no unified standard for
assessing these tools, however, a researcher is faced with difficult questions. Which FPs are
best for general use? Which are the brightest? What additional factors determine which are
best for a given experiment? Although in many cases, a trial-and-error approach may still be
necessary in determining the answers to these questions, a unified characterization of the …
Abstract
The recent explosion in the diversity of available fluorescent proteins (FPs),,,,,,,,,,,,,,, promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPs are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPs provides a useful guide in narrowing down the options.
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