[HTML][HTML] 11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors
Z Ablonczy, RK Crouch, PW Goletz… - Journal of Biological …, 2002 - ASBMB
Rpe65−/− mice produce minimal amounts of 11-cis-retinal, the ligand necessary for the
formation of photosensitive visual pigments. Therefore, the apoprotein opsin in these
animals has not been exposed to its normal ligand. The Rpe65−/− mice contain less than
0.1% of wild type levels of rhodopsin. Mass spectrometric analysis of opsin from Rpe65−/−
mice revealed unusually high levels of phosphorylation in dark-adapted mice but no other
structural alterations. Single flash and flicker electroretinograms (ERGs) from 1-month-old …
formation of photosensitive visual pigments. Therefore, the apoprotein opsin in these
animals has not been exposed to its normal ligand. The Rpe65−/− mice contain less than
0.1% of wild type levels of rhodopsin. Mass spectrometric analysis of opsin from Rpe65−/−
mice revealed unusually high levels of phosphorylation in dark-adapted mice but no other
structural alterations. Single flash and flicker electroretinograms (ERGs) from 1-month-old …