Proteins associated with autosomal dominant and autosomal recessive polycystic kidney
disease (polycystin-1, polycystin-2, and fibrocystin) localize to various subcellular
compartments, but their functional site is thought to be on primary cilia. PC1+ vesicles
surround cilia in Pkhd1 del2/del2 mice, which led us to analyze these structures in detail. We
subfractionated urinary exosome-like vesicles (ELVs) and isolated a subpopulation
abundant in polycystin-1, fibrocystin (in their cleaved forms), and polycystin-2. This removed …