Stimulation of free radical formation by aminoglycoside antibiotics

SH Sha, J Schacht - Hearing research, 1999 - Elsevier
We have previously shown gentamicin to form a redox-active iron chelate. This study
investigates whether other aminoglycosides can likewise stimulate the generation of
reactive oxygen species (free radicals). Kanamycin, neomycin and streptomycin were
compared to gentamicin in intact cells and in cell-free in vitro assays using luminescence
detection with lucigenin or luminol. Neutrophils and Epstein-Barr virus-transformed
lymphoblastoid cells served as cell models in which a respiratory burst of superoxide was …