Objective: To identify the 68-kd target of antibody in serum samples from patients with
idiopathic, progressive, bilateral sensorineural hearing loss. Design: To purify target protein
from renal extracts using gel filtration, ion-exchange chromatography, and poly-acrylamide
gel electrophoresis and to transfer to nitrocellulose membranes. The purified protein was
digested with trypsin, and peptide fragments were separated by high-pressure liquid
chromatography. Results: One fraction obtained by high-performance liquid chromatography …