Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone
DV Goeddel, HL Heyneker, T Hozumi, R Arentzen… - Nature, 1979 - nature.com
DV Goeddel, HL Heyneker, T Hozumi, R Arentzen, K Itakura, DG Yansura, MJ Ross…
Nature, 1979•nature.comDNA coding for human growth hormone was constructed by using chemically synthesised
DNA in conjunction with enzymatically prepared cDNA. This 'hybrid'gene was expressed in
Escherichia coli under the control of the lac promoter. A polypeptide was produced having
the size and immunological properties characteristic of mature human growth hormone.
DNA in conjunction with enzymatically prepared cDNA. This 'hybrid'gene was expressed in
Escherichia coli under the control of the lac promoter. A polypeptide was produced having
the size and immunological properties characteristic of mature human growth hormone.
Abstract
DNA coding for human growth hormone was constructed by using chemically synthesised DNA in conjunction with enzymatically prepared cDNA. This ‘hybrid’ gene was expressed in Escherichia coli under the control of the lac promoter. A polypeptide was produced having the size and immunological properties characteristic of mature human growth hormone.
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