In audiological “toughening” or “conditioning” phenomenon prior exposure to moderate noise reduces the extent of hearing deterioration caused by the subsequent exposure to traumatic test noise known to cause inner ear damage. “Toughening” has been demonstrated in many mammalian laboratory animals such as guinea pig and chinchilla but not in rat or mouse. Our aim was to study the occurrence of this phenomenon in the rat. Ninety-one white male Wistar rats were divided into four groups: unexposed control group (U. n = 10), “conditioning” only (C, n = 32), “conditioning” plus test noise (C + T, n = 36) and test noise only (T, n = 13). Groups C and C + T were “conditioned” for 10 hours with 4.0 kHz OBN between 55 and 95 dB sound pressure levels (SPLs). After 10 hours rest groups C + T and T were exposed to the same noise at 105 dB SPL for 13 hours. The hearing thresholds were determined by auditory brainstem response audiometry (ABR) either immediately after or 3 weeks after the exposures. After that the animals were sacrificed. The cochleas were removed and perilymphatically fixed and further processed for quantitative cytocochleograms. Both the temporary (TTS) and the permanent threshold shifts (PTS) were smaller in animals which had been “conditioned” prior exposure to traumatic noise. Yet only 95 dB SPL “conditioning” gave statistically significant difference (p < 0.05) in PTS. From our results we conclude that “conditioning” effect seems to be present also in the rat. However to confirm this, further experiments are needed. The mechanisms behind “conditioning” are still unknown and also to clarify them, further efforts are needed.