Assessment of intracellular insulin content during all steps of human islet isolation procedure
H Brandhorst, D Brandhorst, MD Brendel, BJ Hering… - Cell Transplantation, 1998 - Elsevier
This study investigated the recovery of pancreatic insulin content during human islet
isolation prior to and after digestion-filtration, continuous Hanks-Ficoll gradient purification
(n= 20), and 3–4 day culture at 22° C (n= 6). The native insulin content varied in a wide
range from 28.4 U to 360.8 U/pancreas. After digestion the initially measured average
insulin content of 115.8±20.8 U/pancreas (mean±SEM) increased to 264.6±22.8%(p<
0.001). This increase of insulin during pancreas digestion was attributed to the asymetrical …
isolation prior to and after digestion-filtration, continuous Hanks-Ficoll gradient purification
(n= 20), and 3–4 day culture at 22° C (n= 6). The native insulin content varied in a wide
range from 28.4 U to 360.8 U/pancreas. After digestion the initially measured average
insulin content of 115.8±20.8 U/pancreas (mean±SEM) increased to 264.6±22.8%(p<
0.001). This increase of insulin during pancreas digestion was attributed to the asymetrical …