[HTML][HTML] Digestion of Streptococcus pneumoniae cell walls with its major peptidoglycan hydrolase releases branched stem peptides carrying proinflammatory activity
PA Majcherczyk, H Langen, D Heumann… - Journal of Biological …, 1999 - ASBMB
The peptidoglycan of Gram-positive bacteria is known to trigger cytokine release from
peripheral blood mononuclear cells (PBMCs). However, it requires 100–1000 times more
Gram-positive peptidoglycan than Gram-negative lipopolysaccharide to release the same
amounts of cytokines from target cells. Thus, either peptidoglycan is poorly active or only
part of it is required for PBMC activation. To test this hypothesis, purified Streptococcus
pneumoniaewalls were digested with their major autolysinN-acetylmuramoyl-l-alanine …
peripheral blood mononuclear cells (PBMCs). However, it requires 100–1000 times more
Gram-positive peptidoglycan than Gram-negative lipopolysaccharide to release the same
amounts of cytokines from target cells. Thus, either peptidoglycan is poorly active or only
part of it is required for PBMC activation. To test this hypothesis, purified Streptococcus
pneumoniaewalls were digested with their major autolysinN-acetylmuramoyl-l-alanine …
