Critical evaluation of cytosolic calcium determination in resting muscle fibres from normal and dystrophic (mdx) mice
P Gailly, B Boland, B Himpens, R Casteels, JM Gillis - Cell calcium, 1993 - Elsevier
The fluorescent probe Fura-2 AM was used to determine cytosolic free calcium
concentration in soleus muscle and in isolated flexor digitorum brevis fibres. This required a
precise calibration; therefore, each calibration parameter was studied in situ. The influence
of the dye concentration on calcium measurements was also examined. This precise
calibration technique was used to compare absolute free calcium concentration in resting
preparations from dystrophic (mdx) and control (C57) mice. We showed that the behavior of …
concentration in soleus muscle and in isolated flexor digitorum brevis fibres. This required a
precise calibration; therefore, each calibration parameter was studied in situ. The influence
of the dye concentration on calcium measurements was also examined. This precise
calibration technique was used to compare absolute free calcium concentration in resting
preparations from dystrophic (mdx) and control (C57) mice. We showed that the behavior of …