Neuronal nitric oxide synthase (NOS) regulates leukocyte‐endothelial cell interactions in endothelial NOS deficient mice
MJ Sanz, MJ Hickey, B Johnston… - British journal of …, 2001 - Wiley Online Library
British journal of pharmacology, 2001•Wiley Online Library
The present study was designed to examine the possible role of neuronal nitric oxide
synthase (nNOS) in regulation of leukocyte–endothelial cell interactions in the absence of
endothelial nitric oxide synthase (eNOS), using intravital microscopy of the cremasteric
microcirculation of eNOS−/− mice. Baseline leukocyte rolling and adhesion revealed no
differences between wild‐type and eNOS−/− mice in either the cremasteric or intestinal
microcirculations. Superfusion with l‐NAME (100 μm) caused a progressive and significant …
synthase (nNOS) in regulation of leukocyte–endothelial cell interactions in the absence of
endothelial nitric oxide synthase (eNOS), using intravital microscopy of the cremasteric
microcirculation of eNOS−/− mice. Baseline leukocyte rolling and adhesion revealed no
differences between wild‐type and eNOS−/− mice in either the cremasteric or intestinal
microcirculations. Superfusion with l‐NAME (100 μm) caused a progressive and significant …
- The present study was designed to examine the possible role of neuronal nitric oxide synthase (nNOS) in regulation of leukocyte – endothelial cell interactions in the absence of endothelial nitric oxide synthase (eNOS), using intravital microscopy of the cremasteric microcirculation of eNOS−/− mice.
- Baseline leukocyte rolling and adhesion revealed no differences between wild‐type and eNOS−/− mice in either the cremasteric or intestinal microcirculations.
- Superfusion with L‐NAME (100 μM) caused a progressive and significant increase in leukocyte adhesion in both wild‐type and eNOS−/− mice, without detecting differences between the two strains of mice.
- Superfusion with 7‐nitroindazole (100 μM), a selective inhibitor of nNOS, had no effect on leukocyte adhesion in wild‐type animals. However, it increased leukocyte adhesion significantly in eNOS−/− mice, which was reversed by systemic L‐arginine pre‐administration.
- Stimulation of the microvasculature with H2O2 (100 μM) induced a transient elevation in leukocyte rolling in wild‐type mice. Conversely, the effect persisted during the entire 60 min of experimental protocol in eNOS−/− mice either with or without 7‐nitroindazole.
- Semi‐quantitative analysis by RT – PCR of the mRNA for nNOS levels in eNOS−/− and wild‐type animals, showed increased expression of nNOS in both brain and skeletal muscle of eNOS−/− mice.
- In conclusion, we have demonstrated that leukocyte‐endothelial cell interactions are predominantly modulated by eNOS isoform in postcapillary venules of normal mice, whereas nNOS appears to assume the same role in eNOS−/− mice. Interestingly, unlike eNOS there was insufficient NO produced by nNOS to overcome leukocyte recruitment elicited by oxidative stress, suggesting that nNOS cannot completely compensate for eNOS.
British Journal of Pharmacology (2001) 134, 305–312; doi:10.1038/sj.bjp.0704234
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