Experimental autoimmune encephalomyelitis in mice: immunologic response to mouse spinal cord and myelin basic proteins
CCA Bernard, PR Carnegie - The Journal of Immunology, 1975 - journals.aai.org
CCA Bernard, PR Carnegie
The Journal of Immunology, 1975•journals.aai.orgIt was confirmed that experimental autoimmune encephalomyelitis EAE, could be induced in
SJL/J mice with mouse spinal cord homogenate. It was shown that induction of EAE in mice
was critically dependent on the concentration of pertussis vaccine. The encephalitogen
present in mouse brain was the basic protein of myelin. The smaller form of the mouse and
rat basic proteins induced EAE; thus the mouse like the rat responds to determinants other
than the “tryptophan region,” which induces EAE in guinea-pigs. Mice with EAE developed a …
SJL/J mice with mouse spinal cord homogenate. It was shown that induction of EAE in mice
was critically dependent on the concentration of pertussis vaccine. The encephalitogen
present in mouse brain was the basic protein of myelin. The smaller form of the mouse and
rat basic proteins induced EAE; thus the mouse like the rat responds to determinants other
than the “tryptophan region,” which induces EAE in guinea-pigs. Mice with EAE developed a …
Abstract
It was confirmed that experimental autoimmune encephalomyelitis EAE, could be induced in SJL/J mice with mouse spinal cord homogenate. It was shown that induction of EAE in mice was critically dependent on the concentration of pertussis vaccine. The encephalitogen present in mouse brain was the basic protein of myelin. The smaller form of the mouse and rat basic proteins induced EAE; thus the mouse like the rat responds to determinants other than the “tryptophan region,” which induces EAE in guinea-pigs.
Mice with EAE developed a cell-mediated immune response to myelin basic protein, as judged by inhibition of peritoneal cell migration. However, levels of antibody to mouse basic protein were low, as judged by radioimmunoassay. The establishment of this autoimmune disease model in the mouse will allow the application of well established techniques for the analysis of the immunologic mechanisms leading to disease manifestation.
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