[HTML][HTML] Characterization of the hematopoietic transcription factor NF-E2 in primary murine megakaryocytes
P Lecine, V Blank, R Shivdasani - Journal of Biological Chemistry, 1998 - ASBMB
Biochemical analysis of megakaryocytes, the precursors of blood platelets, is limited by their
rarity in vivo, and studies on lineage-specific gene expression have been conducted
exclusively in cell lines with limited megakaryocytic potential. Mice lacking the transcription
factor NF-E2 display arrested megakaryocyte differentiation and profound
thrombocytopenia. To study the heterodimeric NF-E2 protein in primary cells, we cultured
mouse fetal livers with the c-Mpl ligand, obtained highly enriched megakaryocyte …
rarity in vivo, and studies on lineage-specific gene expression have been conducted
exclusively in cell lines with limited megakaryocytic potential. Mice lacking the transcription
factor NF-E2 display arrested megakaryocyte differentiation and profound
thrombocytopenia. To study the heterodimeric NF-E2 protein in primary cells, we cultured
mouse fetal livers with the c-Mpl ligand, obtained highly enriched megakaryocyte …