Rab8‐optineurin‐myosin VI: analysis of interactions and functions in the secretory pathway
MV Chibalina, RC Roberts, SD Arden… - Methods in …, 2008 - Elsevier
Abstract The small GTPase Rab8 has been shown to regulate polarized membrane
trafficking pathways from the TGN to the cell surface. Optineurin is an effector protein of
Rab8 and a binding partner of the actin‐based motor protein myosin VI. We used various
approaches to study the interactions between myosin VI and its binding partners and to
analyze their role (s) in intracellular membrane trafficking pathways. In this chapter, we
describe the use of the mammalian two‐hybrid assay to demonstrate protein–protein …
trafficking pathways from the TGN to the cell surface. Optineurin is an effector protein of
Rab8 and a binding partner of the actin‐based motor protein myosin VI. We used various
approaches to study the interactions between myosin VI and its binding partners and to
analyze their role (s) in intracellular membrane trafficking pathways. In this chapter, we
describe the use of the mammalian two‐hybrid assay to demonstrate protein–protein …