MATERIALS AND METHODS MATERIALS: Acrylamide, bisacrylamide, ammonium
persulphate, N, N, N'-tetramethylethylenediamine(TEMED), Coomassie Blue R-250, Trizma
base, sodium dodecyl sulphate (SDS), poly (D-Lysine)(mol. wt. 190,000), Tween 80 and o-
chymotrypsin were purchased from Sigma. Casein (Hammarsten) was from BDH. Human
Clu-plasminogen was from Biopool. Tissue plasminogen activators were from Biopool, UK
National Institute for Biological Standards or in-house preparations produced by …