Purification and characterization of a brain-specific multifunctional calmodulin-dependent protein kinase from rat cerebellum.
O Miyano, I Kameshita, H Fujisawa - Journal of Biological Chemistry, 1992 - ASBMB
A brain-specific multifunctional calmodulin-dependent protein kinase, calmodulin-
dependent protein kinase IV, which exhibited characteristic properties quite different from
those of calmodulin-dependent protein kinase II, was purified approximately 230-fold from
rat cerebellum. The purified preparation gave two protein bands with molecular weights of
63,000 (alpha) and 66,000 (beta) on sodium dodecyl sulfate-polyacrylamide gel
electrophoresis, both of which showed protein kinase activity as examined by the activity gel …
dependent protein kinase IV, which exhibited characteristic properties quite different from
those of calmodulin-dependent protein kinase II, was purified approximately 230-fold from
rat cerebellum. The purified preparation gave two protein bands with molecular weights of
63,000 (alpha) and 66,000 (beta) on sodium dodecyl sulfate-polyacrylamide gel
electrophoresis, both of which showed protein kinase activity as examined by the activity gel …