Organotypic cultures of autologous hair follicle keratinocytes for the treatment of recurrent leg ulcers
A Limat, LE French, L Blal, JH Saurat… - Journal of the American …, 2003 - Elsevier
Our purpose was to evaluate, in an open study, the efficacy of epidermal equivalents (EEs),
a tissue-engineered epidermis prepared from autologous hair follicle keratinocytes, for the
treatment of recurrent leg ulcers (n= 50). To generate EEs, keratinocytes expanded from the
outer root sheaths of plucked anagen hair follicles were seeded on cell culture inserts at air-
liquid interface. The total culture time was 5 to 6 weeks. Three days after the procedure, 95%
of EEs adhered to the wound bed. After 8 weeks, 70% of the total wound surface was re …
a tissue-engineered epidermis prepared from autologous hair follicle keratinocytes, for the
treatment of recurrent leg ulcers (n= 50). To generate EEs, keratinocytes expanded from the
outer root sheaths of plucked anagen hair follicles were seeded on cell culture inserts at air-
liquid interface. The total culture time was 5 to 6 weeks. Three days after the procedure, 95%
of EEs adhered to the wound bed. After 8 weeks, 70% of the total wound surface was re …