An isolation procedure for functionally active lamina propria lymphoid cells (LPL) from the
murine intestine is described. The procedure involved EDTA-dithiothreitol incubation of
intestinal tissue to remove epithelial and intraepithelial cells, followed by collagenase
digestion of the basement membrane to liberate part of the LPL. The LPL were suspended
by squeezing the remaining tissue strips through a nylon gauze filter. Functional activity was
tested by enumeration of the immunoglobulin-secreting cells in the cell suspensions …