[HTML][HTML] Orexins acting at native OX1 receptor in colon cancer and neuroblastoma cells or at recombinant OX1 receptor suppress cell growth by inducing apoptosis
P Rouet-Benzineb, C Rouyer-Fessard, A Jarry… - Journal of Biological …, 2004 - ASBMB
Screening of 26 gut peptides for their ability to inhibit growth of human colon cancer HT29-
D4 cells grown in 10% fetal calf serum identified orexin-A and orexin-B as anti-growth
factors. Upon addition of either orexin (1 μm), suppression of cell growth was total after 24 h
and> 70% after 48 or 72 h, with an EC 50 of 5 nm peptide. Orexins did not alter proliferation
but promoted apoptosis as demonstrated by morphological changes in cell shape, DNA
fragmentation, chromatin condensation, cytochrome c release into cytosol, and activation of …
D4 cells grown in 10% fetal calf serum identified orexin-A and orexin-B as anti-growth
factors. Upon addition of either orexin (1 μm), suppression of cell growth was total after 24 h
and> 70% after 48 or 72 h, with an EC 50 of 5 nm peptide. Orexins did not alter proliferation
but promoted apoptosis as demonstrated by morphological changes in cell shape, DNA
fragmentation, chromatin condensation, cytochrome c release into cytosol, and activation of …
