[HTML][HTML] IκB kinases phosphorylate NF-κB p65 subunit on serine 536 in the transactivation domain
H Sakurai, H Chiba, H Miyoshi, T Sugita… - Journal of Biological …, 1999 - ASBMB
Recent investigations have elucidated the cytokine-induced NF-κB activation pathway. IκB
kinase (IKK) phosphorylates inhibitors of NF-κB (IκBs). The phosphorylation targets them for
rapid degradation through a ubiquitin-proteasome pathway, allowing the nuclear
translocation of NF-κB. We have examined the possibility that IKK can phosphorylate the
p65 NF-κB subunit as well as IκB in the cytokine-induced NF-κB activation. In the cytoplasm
of HeLa cells, the p65 subunit was rapidly phosphorylated in response to TNF-α in a time …
kinase (IKK) phosphorylates inhibitors of NF-κB (IκBs). The phosphorylation targets them for
rapid degradation through a ubiquitin-proteasome pathway, allowing the nuclear
translocation of NF-κB. We have examined the possibility that IKK can phosphorylate the
p65 NF-κB subunit as well as IκB in the cytokine-induced NF-κB activation. In the cytoplasm
of HeLa cells, the p65 subunit was rapidly phosphorylated in response to TNF-α in a time …