A new method for removal of mononuclear phagocytes from heterogeneous cell populations in vitro, using the liposome-mediated macrophage 'suicide'technique
I Claassen, N Van Rooijen, E Claassen - Journal of immunological …, 1990 - Elsevier
In this study we present a new method for the elimination of mononuclear phagocytic cells
from cell suspensions. By making use of liposome-encapsulated dichloromethylene
diphosphonate we were able to effectively remove macrophages from spleen cell
suspensions. This effect was not observed when using the free drug or control (PBS)
liposomes. The use of this procedure has no effect on other cell types, as measured by
growth, protein production, antigen presentation and antigen specific T cell proliferation …
from cell suspensions. By making use of liposome-encapsulated dichloromethylene
diphosphonate we were able to effectively remove macrophages from spleen cell
suspensions. This effect was not observed when using the free drug or control (PBS)
liposomes. The use of this procedure has no effect on other cell types, as measured by
growth, protein production, antigen presentation and antigen specific T cell proliferation …