[HTML][HTML] Affinity and kinetic analysis of P-selectin binding to P-selectin glycoprotein ligand-1
P Mehta, RD Cummings, RP McEver - Journal of Biological Chemistry, 1998 - ASBMB
Leukocytes use the cell-surface mucin P-selectin glycoprotein ligand-1 (PSGL-1) to tether to
and roll on P-selectin on activated endothelial cells and platelets. By using surface plasmon
resonance, we measured the affinity and kinetics of binding of soluble monomeric human P-
selectin to immobilized PSGL-1 from human neutrophils. Binding was specific, as
documented by its Ca 2+-dependence, its inhibition by specific monoclonal antibodies to P-
selectin and PSGL-1, and its abrogation by treating PSGL-1 with sialidase. Similar binding …
and roll on P-selectin on activated endothelial cells and platelets. By using surface plasmon
resonance, we measured the affinity and kinetics of binding of soluble monomeric human P-
selectin to immobilized PSGL-1 from human neutrophils. Binding was specific, as
documented by its Ca 2+-dependence, its inhibition by specific monoclonal antibodies to P-
selectin and PSGL-1, and its abrogation by treating PSGL-1 with sialidase. Similar binding …