Recombinant human aggrecan G1-G2 exhibits native binding properties and substrate specificity for matrix metalloproteinases and aggrecanase
FA Mercuri, KJ Doege, EC Arner, MA Pratta… - Journal of Biological …, 1999 - ASBMB
A recombinant human aggrecan G1-G2 fragment comprising amino acids Val 1-Arg 656 has
been expressed in Sf21 cells using a baculovirus expression system. The recombinant G1-
G2 (rG1-G2) was purified to homogeneity by hyaluronan-Sepharose affinity chromatography
followed by high performance liquid chromatography gel filtration, and gave a single band of
M r 90,000–95,000 by silver stain or immunoblotting with monoclonal antibody 1-C-6. The
expressed G1-G2 bound to both hyaluronan and link protein indicating that the …
been expressed in Sf21 cells using a baculovirus expression system. The recombinant G1-
G2 (rG1-G2) was purified to homogeneity by hyaluronan-Sepharose affinity chromatography
followed by high performance liquid chromatography gel filtration, and gave a single band of
M r 90,000–95,000 by silver stain or immunoblotting with monoclonal antibody 1-C-6. The
expressed G1-G2 bound to both hyaluronan and link protein indicating that the …
