Ex vivo analysis of human antigen-specific CD8+ T-cell responses: quality assessment of fluorescent HLA-A2 multimer and interferon-γ ELISPOT assays for patient …
DE Speiser, MJ Pittet, P Guillaume… - Journal of …, 2004 - journals.lww.com
The authors developed a standardized approach for immune monitoring of antigen-specific
CD8+ T cells within peripheral blood lymphocytes (PBLs) that combines direct ex vivo
analysis of Melan-A/MART-1 and influenza-specific CD8+ T cells with HLA-A2/peptide
multimers and interferon-γ ELISPOT assays. Here the authors assessed the quality of results
obtained with 180 PBLs from healthy donors and melanoma patients. Reproducibility of the
multimer assay was good (average of 15% variation). In the absence of in vivo antigen …
CD8+ T cells within peripheral blood lymphocytes (PBLs) that combines direct ex vivo
analysis of Melan-A/MART-1 and influenza-specific CD8+ T cells with HLA-A2/peptide
multimers and interferon-γ ELISPOT assays. Here the authors assessed the quality of results
obtained with 180 PBLs from healthy donors and melanoma patients. Reproducibility of the
multimer assay was good (average of 15% variation). In the absence of in vivo antigen …