Antigen-specific immunoadsorption of pathogenic autoantibodies in pemphigus foliaceus

M Amagai, T Hashimoto, KJ Green, N Shimizu… - Journal of Investigative …, 1995 - Elsevier
M Amagai, T Hashimoto, KJ Green, N Shimizu, T Nishikawa
Journal of Investigative Dermatology, 1995Elsevier
Patients with the autoimmune blistering disease pemphigus foliaceus (PF) have circulating
autoantibodies directed against the desmosomal cadherin desmoglein 1 (Dsg1). Based on
the fact that purified PG fractions from PF patients induce loss of cell adhesion in organ
culture and in a neonatal mouse model, it has been proposed that these anti-Dsg1
antibodies play a pathogenic role in blister formation. To directly address whether antibodies
in PF sera specific for the Dsg1 extracellular domain are indeed pathogenic in the disease …
Patients with the autoimmune blistering disease pemphigus foliaceus (PF) have circulating autoantibodies directed against the desmosomal cadherin desmoglein 1 (Dsg1). Based on the fact that purified PG fractions from PF patients induce loss of cell adhesion in organ culture and in a neonatal mouse model, it has been proposed that these anti-Dsg1 antibodies play a pathogenic role in blister formation. To directly address whether antibodies in PF sera specific for the Dsg1 extracellular domain are indeed pathogenic in the disease, PFIg, a chimeric protein containing the entire extracellular domain of human Dsg1 and the constant region of human IgG1, was produced by baculovirus expression. Incubation of PF patients' sera with the PFIg baculoprotein removed the immunoreactivity of autoantibodies against keratinocyte cell surfaces in all 20 PF and eight Brazilian PF patients' sera tested. This absorption was conformation dependent, because PFIg protein denatured by low pH or heat was no longer able to adsorb the immunoreactivity of PF sera. Furthermore, tile incubation with the PEIg baculoprotein eliminated the pathogenic activity of PF patients' sera and prevented gross blister formation in a neonatal mouse model of pemphigus. Anti-Dsg1 anti-bodies eluted from the PFIg protein column were pathogenic as they resulted in the appearance of gross blisters in neonatal mice with typical histologic findings of PF. These observations indicate that the extracellular domain of Dsg1 expressed by baculovirus is capable of specifically Immunoadsorbing pathogenic autoantibodies front PF patients' sera and provide direct evidence that the anti-Dsg1 auto-antibodies in PF sera are indeed pathogenic. The availability of this Dsg1 recombinant protein may facilitate the development of antigen-specific plasma-pheresis as a novel therapeutic strategy in pemphigus.
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