The major goal of this work is to establish a culture system for the growth of human B
lymphocytes at the single-cell level so that the immunoglobulin secreted by the clonal
progeny of that cell can be analyzed. A method which involves culturing small numbers (1–
1000) of lymphocytes, which have been infected with Epstein-Barr virus (EBV) prior to
plating, in round-bottom microtiter plates is described. A feeder layer of irradiated (2500 R)
umbilical cord blood lymphocytes to which phytohemagglutinin has been added was found …