Escherichia coli ribonuclease III: affinity purification of hexahistidine-tagged enzyme and assays for substrate binding and cleavage
AK Amarasinghe, I Calin-Jageman, A Harmouch… - Methods in …, 2001 - Elsevier
It is now evident that members of the RNase III family of nucleases have central roles in
prokaryotic and eukaryotic RNA maturation and decay pathways. Ongoing research is
uncovering new roles for RNase III homologs. For example, the phenomena of RNA
interference (RNAi) and posttranscriptional gene silencing (PTGS) involve dsRNA
processing, carried out by an RNase III homolog. We anticipate an increased focus on the
mechanism, regulation, and biological roles of RNase III orthologs. Although the differences …
prokaryotic and eukaryotic RNA maturation and decay pathways. Ongoing research is
uncovering new roles for RNase III homologs. For example, the phenomena of RNA
interference (RNAi) and posttranscriptional gene silencing (PTGS) involve dsRNA
processing, carried out by an RNase III homolog. We anticipate an increased focus on the
mechanism, regulation, and biological roles of RNase III orthologs. Although the differences …