[HTML][HTML] Activation of an H2O2-generating NADH Oxidase in Human Lung Fibroblasts by Transforming Growth Factor β1 (∗)
VJ Thannickal, BL Fanburg - Journal of Biological Chemistry, 1995 - ASBMB
The cellular source (s) and mechanisms of generation of reactive oxygen species (ROS) in
nonphagocytic cells stimulated by cytokines are unclear. In this study, we demonstrate that
transforming growth factor β1 (TGF-β1, 1 ng/ml) induces the release of H 2 O 2 from human
lung fibroblasts within 8 h following exposure to this cytokine. Elevation in H 2 O 2 release
peaked at 16 h (∼ 22 pmol/min/10 6 cells) and gradually declined to undetectable levels at
48 h after TGF-β1 treatment. NADH consumption by these cells was stimulated by TGF-β1 …
nonphagocytic cells stimulated by cytokines are unclear. In this study, we demonstrate that
transforming growth factor β1 (TGF-β1, 1 ng/ml) induces the release of H 2 O 2 from human
lung fibroblasts within 8 h following exposure to this cytokine. Elevation in H 2 O 2 release
peaked at 16 h (∼ 22 pmol/min/10 6 cells) and gradually declined to undetectable levels at
48 h after TGF-β1 treatment. NADH consumption by these cells was stimulated by TGF-β1 …