[PDF][PDF] Modification of dengue virus strains by passage in primary dog kidney cells: preparation of candidate vaccines and immunization of monkeys

KH Eckels, DR Dubois, R Putnak… - American Journal of …, 2003 - researchgate.net
KH Eckels, DR Dubois, R Putnak, DW Vaughn, BL Innis, EA Henchal, CH Hoke Jr
American Journal of Tropical Medicine and Hygiene, 2003researchgate.net
Dengue (DENV) virus strains for each of the four DENV serotypes were modified by passage
in primary dog kidney (PDK) cell cultures with final manufacture of vaccine lots in fetal
rhesus monkey diploid cell cultures.“Strain sets” consisting of serially-passaged DENV were
inoculated in rhesus monkeys along with unmodified parent viruses for each strain. Vaccine
candidates were compared with unmodified parent viruses by measuring viremia and
immune responses. All except one DENV-1 strain demonstrated reduced infection in …
Abstract
Dengue (DENV) virus strains for each of the four DENV serotypes were modified by passage in primary dog kidney (PDK) cell cultures with final manufacture of vaccine lots in fetal rhesus monkey diploid cell cultures.“Strain sets” consisting of serially-passaged DENV were inoculated in rhesus monkeys along with unmodified parent viruses for each strain. Vaccine candidates were compared with unmodified parent viruses by measuring viremia and immune responses. All except one DENV-1 strain demonstrated reduced infection in monkeys after PDK cell passage. A DENV-3 strain lost all monkey infectivity after PDK cell passage. Twelve vaccine candidates were selected for Phase 1 human trials through this selection process.
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