Targeted derepression of the human immunodeficiency virus type 1 long terminal repeat by pyrrole-imidazole polyamides
JJ Coull, G He, C Melander, VC Rucker… - Journal of …, 2002 - Am Soc Microbiol
JJ Coull, G He, C Melander, VC Rucker, PB Dervan, DM Margolis
Journal of virology, 2002•Am Soc MicrobiolThe host factor LSF represses the human immunodeficiency virus type 1 long terminal
repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-
imidazole polyamides targeted to the LTR can specifically block LSF binding both in vitro
and within cells via direct access to chromatin, resulting in increased LTR expression.
repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-
imidazole polyamides targeted to the LTR can specifically block LSF binding both in vitro
and within cells via direct access to chromatin, resulting in increased LTR expression.
Abstract
The host factor LSF represses the human immunodeficiency virus type 1 long terminal repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-imidazole polyamides targeted to the LTR can specifically block LSF binding both in vitro and within cells via direct access to chromatin, resulting in increased LTR expression.
American Society for Microbiology