[HTML][HTML] Post-translational processing of RhoA: carboxyl methylation of the carboxyl-terminal prenylcysteine increases the half-life of RhoA
PS Backlund - Journal of Biological Chemistry, 1997 - ASBMB
RhoA and related GTP-binding proteins are modified post-translationally at their carboxyl
terminus to form a prenylcysteine methyl ester. The synthesis and post-translational
modification of RhoA and Cdc42 were examined in the RAW264 macrophage cell line, and
the effect of carboxyl methylation on protein turnover was determined. Cells were labeled
with [35 S] cysteine, and RhoA or Cdc42 was immunoprecipitated with specific antibodies.
Both RhoA and Cdc42 were methylated rapidly in control cells, with little accumulation of …
terminus to form a prenylcysteine methyl ester. The synthesis and post-translational
modification of RhoA and Cdc42 were examined in the RAW264 macrophage cell line, and
the effect of carboxyl methylation on protein turnover was determined. Cells were labeled
with [35 S] cysteine, and RhoA or Cdc42 was immunoprecipitated with specific antibodies.
Both RhoA and Cdc42 were methylated rapidly in control cells, with little accumulation of …