[PDF][PDF] Characterization of the alloimmune response to minor histocompatibility antigens by in vivo MLR
HK Song, H Noorchashm, YK Lieu… - Transplantation …, 1999 - academia.edu
HK Song, H Noorchashm, YK Lieu, S Rostami, SAS Greeley, CF Barker, A Naji
Transplantation proceedings, 1999•academia.eduMATERIALS AND METHODS Responder lymphocytes from C57BL/6 (H-2b) responder
strain mice were isolated, labeled with CFSE as previously described, and adoptively
transferred into lethally irradiated C3H/HeJ (H-2k) or congenic C3H. SW-H-2bSnJ (H-2b)
stimulator strain mice (30 million responder cells/recipient). 4 Lethally irradiated stimulator
C57BL/6 mice were used as syngeneic controls. Responder lymphocytes were harvested
from stimulator mice at serial time points, incubated with phycoerythrin-conjugated anti-CD4 …
strain mice were isolated, labeled with CFSE as previously described, and adoptively
transferred into lethally irradiated C3H/HeJ (H-2k) or congenic C3H. SW-H-2bSnJ (H-2b)
stimulator strain mice (30 million responder cells/recipient). 4 Lethally irradiated stimulator
C57BL/6 mice were used as syngeneic controls. Responder lymphocytes were harvested
from stimulator mice at serial time points, incubated with phycoerythrin-conjugated anti-CD4 …
MATERIALS AND METHODS
Responder lymphocytes from C57BL/6 (H-2b) responder strain mice were isolated, labeled with CFSE as previously described, and adoptively transferred into lethally irradiated C3H/HeJ (H-2k) or congenic C3H. SW-H-2bSnJ (H-2b) stimulator strain mice (30 million responder cells/recipient). 4 Lethally irradiated stimulator C57BL/6 mice were used as syngeneic controls. Responder lymphocytes were harvested from stimulator mice at serial time points, incubated with phycoerythrin-conjugated anti-CD4 monoclonal antibody (Pharmingen, San Diego, Calif) at 4 for 30 minutes, and analyzed on a FACScan (Becton Dickinson, Mountain View, Calif).
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