[HTML][HTML] Calculation of Absolute Metabolic Flux and the Elucidation of the Pathways of Glutamate Labeling in Perfused Rat Heart by 13C NMR Spectroscopy and …
JC Chatham, JR Forder, JD Glickson… - Journal of Biological …, 1995 - ASBMB
Absolute metabolic fluxes in isolated perfused hearts have been determined by a nonlinear
least squares analysis of glutamate labeling kinetics from [1-13 C] glucose,[4-13 C] β-
hydroxybutyrate, or [2-13 C] acetate using 13 C NMR spectroscopy. With glucose as
substrate, the malate-aspartate shuttle flux was too slow to account for the reducing
equivalents generated by glycolysis and to predict the observed oxygen consumption rate.
For acetate and β-hydroxybutyrate, the malate-aspartate shuttle had to be reversed for the …
least squares analysis of glutamate labeling kinetics from [1-13 C] glucose,[4-13 C] β-
hydroxybutyrate, or [2-13 C] acetate using 13 C NMR spectroscopy. With glucose as
substrate, the malate-aspartate shuttle flux was too slow to account for the reducing
equivalents generated by glycolysis and to predict the observed oxygen consumption rate.
For acetate and β-hydroxybutyrate, the malate-aspartate shuttle had to be reversed for the …