[HTML][HTML] Characterization of chimeric lipopolysaccharides from Escherichia coli strain JM109 transformed with lipooligosaccharide synthesis genes (lsg) from …

NJ Phillips, TJ Miller, JJ Engstrom, W Melaugh… - Journal of Biological …, 2000 - ASBMB
Previously, we reported the expression of chimeric lipopolysaccharides (LPS) in Escherichia
colistrain JM109 (a K-12 strain) transformed with plasmids containingHaemophilus
influenzae lipooligosaccharide synthesis genes (lsg)(Abu Kwaik, Y., McLaughlin, RE,
Apicella, MA, and Spinola, SM (1991) Mol. Microbiol. 5, 2475–2480). In this current study,
we have analyzed theO-deacylated LPS and free oligosaccharides from three transformants
(designated pGEMLOS-4, pGEMLOS-5, and pGEMLOS-7) by matrix-assisted laser …