A surrogate method for assessment of β2-integrin-dependent adhesion of human eosinophils to ICAM-1
X Zhu, R Subbaraman, H Sano, B Jacobs… - Journal of …, 2000 - Elsevier
We have developed and validated an inexpensive and equivalent method for measuring
eosinophil adhesion by β2-integrin to endothelial ICAM-1 using bovine serum albumin
(BSA) as a surrogate for the immunoglobulin supergene. The number of adherent
eosinophils on BSA or ICAM-1 coated microplates was quantified by residual eosinophil
peroxidase activity. Non-stimulated eosinophils did not adhere to either BSA or ICAM-1.
However, after IL-5 stimulation, either BSA or ICAM-1 caused comparable and concentration …
eosinophil adhesion by β2-integrin to endothelial ICAM-1 using bovine serum albumin
(BSA) as a surrogate for the immunoglobulin supergene. The number of adherent
eosinophils on BSA or ICAM-1 coated microplates was quantified by residual eosinophil
peroxidase activity. Non-stimulated eosinophils did not adhere to either BSA or ICAM-1.
However, after IL-5 stimulation, either BSA or ICAM-1 caused comparable and concentration …
