Glomerular epithelial cells secrete a glomerular basement membrane-degrading metalloproteinase.
R Johnson, H Yamabe, YP Chen… - Journal of the …, 1992 - journals.lww.com
Cultured rat glomerular epithelial cells (GEC) were examined for their ability to release
extracellular matrix-degrading proteinases with [3H] gelatin as substrate. GEC-conditioned
media, under serum-free conditions, contained modest amounts of gelatinase activity (1 to
10 U/mg of protein); the activity was maximal at neutral pH, was inhibited by zinc chelators,
was not inhibited by tissue inhibitor of metalloproteinase-2, and could not be further
activated by trypsin or organomercurials. Gelatin substrate sodium dodecyl sulfate …
extracellular matrix-degrading proteinases with [3H] gelatin as substrate. GEC-conditioned
media, under serum-free conditions, contained modest amounts of gelatinase activity (1 to
10 U/mg of protein); the activity was maximal at neutral pH, was inhibited by zinc chelators,
was not inhibited by tissue inhibitor of metalloproteinase-2, and could not be further
activated by trypsin or organomercurials. Gelatin substrate sodium dodecyl sulfate …
