Cloning and expression of murine immune interferon cDNA.
PW Gray, DV Goeddel - Proceedings of the National …, 1983 - National Acad Sciences
PW Gray, DV Goeddel
Proceedings of the National Academy of Sciences, 1983•National Acad SciencesThe murine immune interferon (IFN-gamma) gene was cloned and expressed under control
of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted
from these cells having the biological, antigenic, and biochemical characteristics of natural
murine IFN-gamma. Cloned murine IFN-gamma cDNAs were obtained by using RNA from
both mitogen-induced murine spleens and the transfected COS cells, and both code for
identical proteins. The mature murine IFN-gamma encoded is 136 amino acids long, 10 …
of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted
from these cells having the biological, antigenic, and biochemical characteristics of natural
murine IFN-gamma. Cloned murine IFN-gamma cDNAs were obtained by using RNA from
both mitogen-induced murine spleens and the transfected COS cells, and both code for
identical proteins. The mature murine IFN-gamma encoded is 136 amino acids long, 10 …
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of natural murine IFN-gamma. Cloned murine IFN-gamma cDNAs were obtained by using RNA from both mitogen-induced murine spleens and the transfected COS cells, and both code for identical proteins. The mature murine IFN-gamma encoded is 136 amino acids long, 10 amino acids shorter than human IFN-gamma. The nucleotide homology between the murine and human IFN-gamma genes is 60-65%, whereas the encoded proteins are only 40% homologous. Murine IFN-gamma cDNA was expressed in Escherichia coli under trp promoter control.
National Acad Sciences