Follicular dendritic cells specifically express the long CR2/CD21 isoform
YJ Liu, J Xu, O de Bouteiller, CL Parham… - The Journal of …, 1997 - rupress.org
YJ Liu, J Xu, O de Bouteiller, CL Parham, G Grouard, O Djossou, B de Saint-Vis, S Lebecque…
The Journal of experimental medicine, 1997•rupress.orgThis paper describes an antibody (mAb 7D6) that specifically recognizes human follicular
dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human
CR2/CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We
demonstrated that FDCs selectively express CD21L, while B cells selectively express the
short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk− cells transfected with
the CD21L cDNA, we further showed that the other two anti–human FDC mAbs DRC-1 and …
dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human
CR2/CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We
demonstrated that FDCs selectively express CD21L, while B cells selectively express the
short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk− cells transfected with
the CD21L cDNA, we further showed that the other two anti–human FDC mAbs DRC-1 and …
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk− cells transfected with the CD21L cDNA, we further showed that the other two anti–human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.
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