House dust mite-specific T cells in the skin of subjects with atopic dermatitis: frequency and lymphokine profile in the allergen patch test
N Sager, A Feldmann, G Schilling, P Kreitsch… - Journal of allergy and …, 1992 - Elsevier
The mechanism underlying positive patch tests with house dust mite—allergen,
Dermatophagoides pteronyssinus (Der p), in patients with atopic dermatitis was investigated
by isolating T cells from the test sites of two patients. Eighty-five T cell clones (TCC) were
established from the epidermis and dermis of lesional skin by the limiting-dilution method
with Der p and interleukin (IL)-2. With restimulation assays, 29 of 60 TCCs tested
demonstrated specific proliferation; 85% were of the CD3+, CD2+, and CD4+ phenotype …
Dermatophagoides pteronyssinus (Der p), in patients with atopic dermatitis was investigated
by isolating T cells from the test sites of two patients. Eighty-five T cell clones (TCC) were
established from the epidermis and dermis of lesional skin by the limiting-dilution method
with Der p and interleukin (IL)-2. With restimulation assays, 29 of 60 TCCs tested
demonstrated specific proliferation; 85% were of the CD3+, CD2+, and CD4+ phenotype …