An immunogenetic analysis of T-cell reactive regions on the major allergen from the house dust mite, Der p I, with recombinant truncated fragments
RM O'Brien, WR Thomas, BD Tait - Journal of allergy and clinical …, 1994 - Elsevier
Proliferation assays were used to localize the T-cell reactive sites on the major allergen from
the house dust mite Dermatophagoides pteronyssinus, Der p I. Seven overlapping
recombinant fragments of the Der p I molecule, synthesized with the pGEX expression vector
system, were used to stimulate peripheral blood lymphocytes from 35 HDM-sensitive
individuals. The fusion fragments were from 39 to 114 amino acids in length and spanned
the entire Der p I molecule. Significant proliferative responses to one or more of the …
the house dust mite Dermatophagoides pteronyssinus, Der p I. Seven overlapping
recombinant fragments of the Der p I molecule, synthesized with the pGEX expression vector
system, were used to stimulate peripheral blood lymphocytes from 35 HDM-sensitive
individuals. The fusion fragments were from 39 to 114 amino acids in length and spanned
the entire Der p I molecule. Significant proliferative responses to one or more of the …