[HTML][HTML] Erythrocytes possess an intrinsic barrier to nitric oxide consumption
It has been reported that free hemoglobin (Hb) reacts with NO at an extremely high rate (K
Hb∼ 10 7 m− 1 s− 1) and that the red blood cell (RBC) membrane is highly permeable to
NO. RBCs, however, react with NO 500–1000 times slower. This reduction of NO reaction
rate by RBCs has been attributed to the extracellular diffusion limitation. To test whether
additional limitations are also important, we designed a competition test, which allows the
extracellular diffusion limitation to be distinguished from transmembrane or intracellular …
Hb∼ 10 7 m− 1 s− 1) and that the red blood cell (RBC) membrane is highly permeable to
NO. RBCs, however, react with NO 500–1000 times slower. This reduction of NO reaction
rate by RBCs has been attributed to the extracellular diffusion limitation. To test whether
additional limitations are also important, we designed a competition test, which allows the
extracellular diffusion limitation to be distinguished from transmembrane or intracellular …