Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase.
QW Xie, Y Kashiwabara, C Nathan - Journal of Biological Chemistry, 1994 - ASBMB
The promoter of the murine gene encoding inducible nitric oxide synthase (iNOS) contains
an NF-kappa B site beginning 55 base pairs upstream of the TATA box, designated NF-
kappa Bd. Reporter constructs containing truncated promoter regions, when transfected into
macrophages, revealed that NF-kappa Bd is necessary to confer inducibility by bacterial
lipopolysaccharide (LPS). Oligonucleotide probes containing NF-kappa Bd plus the
downstream 9 or 47 base pairs bound proteins that rapidly appeared in the nuclei of LPS …
an NF-kappa B site beginning 55 base pairs upstream of the TATA box, designated NF-
kappa Bd. Reporter constructs containing truncated promoter regions, when transfected into
macrophages, revealed that NF-kappa Bd is necessary to confer inducibility by bacterial
lipopolysaccharide (LPS). Oligonucleotide probes containing NF-kappa Bd plus the
downstream 9 or 47 base pairs bound proteins that rapidly appeared in the nuclei of LPS …