[HTML][HTML] Hydroperoxide dependence and cooperative cyclooxygenase kinetics in prostaglandin H synthase-1 and-2
W Chen, TR Pawelek, RJ Kulmacz - Journal of Biological Chemistry, 1999 - ASBMB
Prostaglandin H synthase isoform-1 (PGHS-1) cyclooxygenase activity has a cooperative
response to arachidonate concentration, whereas the second isoform, PGHS-2, exhibits
saturable kinetics. The basis for the cooperative PGHS-1 behavior and for the difference in
cooperativity between the isoforms was unclear. The two cyclooxygenase activities have
different efficiencies of feedback activation by hydroperoxide. To determine whether the
cooperative kinetics were governed by the feedback activation characteristics, we examined …
response to arachidonate concentration, whereas the second isoform, PGHS-2, exhibits
saturable kinetics. The basis for the cooperative PGHS-1 behavior and for the difference in
cooperativity between the isoforms was unclear. The two cyclooxygenase activities have
different efficiencies of feedback activation by hydroperoxide. To determine whether the
cooperative kinetics were governed by the feedback activation characteristics, we examined …
