[HTML][HTML] Thrombin induces proteinase-activated receptor-1 gene expression in endothelial cells via activation of Gi-linked Ras/mitogen-activated protein kinase …
We addressed the mechanisms of restoration of cell surface proteinase-activated receptor-1
(PAR-1) by investigating thrombin-activated signaling pathways involved in PAR-1 re-
expression in endothelial cells. Exposure of endothelial cells transfected with PAR-1
promoter-luciferase reporter construct to either thrombin or PAR-1 activating peptide
increased the steady-state PAR-1 mRNA and reporter activity, respectively. Pretreatment of
reporter-transfected endothelial cells with pertussis toxin or co-expression of a minigene …
(PAR-1) by investigating thrombin-activated signaling pathways involved in PAR-1 re-
expression in endothelial cells. Exposure of endothelial cells transfected with PAR-1
promoter-luciferase reporter construct to either thrombin or PAR-1 activating peptide
increased the steady-state PAR-1 mRNA and reporter activity, respectively. Pretreatment of
reporter-transfected endothelial cells with pertussis toxin or co-expression of a minigene …