[CITATION][C] p53, guardian of the genome

DP Lane - Nature, 1992 - nature.com
Untitled Page 1 Page 2 NEWS AND VIEWS tion of p53 with its sequence-specific DNA-binding
function to see if the protein would act as a specific transcription factor in its own right. Several
groups have now taken this step by placing the p53 binding consensus sequence upstream of
a minimal promoter and a reporter gene, and then looking at the effect of p53 on the expression
of the reporter. The experiments work beautifully in vivo, both in yeast and human cells!3,!4.
Only the wild-type protein is active, and again the inhibitory effect of dominant-negative p53 …